Interplay between formation of photosynthetic complexes and expression of genes for iron-sulfur cluster assembly in Rhodobacter sphaeroides?
Identifieur interne : 000098 ( Main/Exploration ); précédent : 000097; suivant : 000099Interplay between formation of photosynthetic complexes and expression of genes for iron-sulfur cluster assembly in Rhodobacter sphaeroides?
Auteurs : Xin Nie [Allemagne, République populaire de Chine] ; Andreas J Ger [Allemagne] ; Janek Börner [Allemagne] ; Gabriele Klug [Allemagne]Source :
- Photosynthesis research [ 1573-5079 ] ; 2020.
Abstract
Formation of photosynthetic complexes leads to a higher demand for Fe-S clusters. We hypothesized that in the facultative phototrophic alpha-proteobacterium Rhodobacter sphaeroides expression of the isc-suf operon for Fe-S cluster formation may be increased under conditions that promote formation of photosynthetic complexes and that, vice versa, lack of the IscR regulator may also affect photosynthesis gene expression. To test this hypothesis, we monitored the activities of the isc-suf sense and anti-sense promoters under different growth conditions and in mutants which are impaired in formation of photosynthetic complexes. We also tested expression of photosynthesis genes in a mutant lacking the IscR regulator. Our results are not in agreement with a co-regulation of the Isc-Suf system and the photosynthetic apparatus at level of transcription. We provide evidence that, coordination of the systems occurs at post-transcriptional levels. Increased levels of isc-suf mRNAs under conditions promoting formation of photosynthetic complexes are due to higher RNA stability.
DOI: 10.1007/s11120-020-00789-w
PubMed: 33064275
Affiliations:
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Gabriele.Klug@mikro.bio.uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Microbiology and Molecular Biology, University of Giessen, IFZ, Heinrich-Buff-Ring, 26-32</wicri:regionArea><wicri:noRegion>26-32</wicri:noRegion><wicri:noRegion>26-32</wicri:noRegion><wicri:noRegion>26-32</wicri:noRegion></affiliation></author></analytic><series><title level="j">Photosynthesis research</title><idno type="eISSN">1573-5079</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Formation of photosynthetic complexes leads to a higher demand for Fe-S clusters. We hypothesized that in the facultative phototrophic alpha-proteobacterium Rhodobacter sphaeroides expression of the isc-suf operon for Fe-S cluster formation may be increased under conditions that promote formation of photosynthetic complexes and that, vice versa, lack of the IscR regulator may also affect photosynthesis gene expression. To test this hypothesis, we monitored the activities of the isc-suf sense and anti-sense promoters under different growth conditions and in mutants which are impaired in formation of photosynthetic complexes. We also tested expression of photosynthesis genes in a mutant lacking the IscR regulator. Our results are not in agreement with a co-regulation of the Isc-Suf system and the photosynthetic apparatus at level of transcription. We provide evidence that, coordination of the systems occurs at post-transcriptional levels. Increased levels of isc-suf mRNAs under conditions promoting formation of photosynthetic complexes are due to higher RNA stability.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33064275</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-5079</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Oct</Month><Day>16</Day></PubDate></JournalIssue><Title>Photosynthesis research</Title><ISOAbbreviation>Photosynth Res</ISOAbbreviation></Journal><ArticleTitle>Interplay between formation of photosynthetic complexes and expression of genes for iron-sulfur cluster assembly in Rhodobacter sphaeroides?</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11120-020-00789-w</ELocationID><Abstract><AbstractText>Formation of photosynthetic complexes leads to a higher demand for Fe-S clusters. We hypothesized that in the facultative phototrophic alpha-proteobacterium Rhodobacter sphaeroides expression of the isc-suf operon for Fe-S cluster formation may be increased under conditions that promote formation of photosynthetic complexes and that, vice versa, lack of the IscR regulator may also affect photosynthesis gene expression. To test this hypothesis, we monitored the activities of the isc-suf sense and anti-sense promoters under different growth conditions and in mutants which are impaired in formation of photosynthetic complexes. We also tested expression of photosynthesis genes in a mutant lacking the IscR regulator. Our results are not in agreement with a co-regulation of the Isc-Suf system and the photosynthetic apparatus at level of transcription. We provide evidence that, coordination of the systems occurs at post-transcriptional levels. Increased levels of isc-suf mRNAs under conditions promoting formation of photosynthetic complexes are due to higher RNA stability.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nie</LastName><ForeName>Xin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Microbiology and Molecular Biology, University of Giessen, IFZ, Heinrich-Buff-Ring, 26-32, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Basic Medical Sciences, Chengdu Medical College, Chengdu, 610500, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jäger</LastName><ForeName>Andreas</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Microbiology and Molecular Biology, University of Giessen, IFZ, Heinrich-Buff-Ring, 26-32, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Börner</LastName><ForeName>Janek</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-4824-9393</Identifier><AffiliationInfo><Affiliation>Institute of Microbiology and Molecular Biology, University of Giessen, IFZ, Heinrich-Buff-Ring, 26-32, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Klug</LastName><ForeName>Gabriele</ForeName><Initials>G</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-3527-5093</Identifier><AffiliationInfo><Affiliation>Institute of Microbiology and Molecular Biology, University of Giessen, IFZ, Heinrich-Buff-Ring, 26-32, Germany. Gabriele.Klug@mikro.bio.uni-giessen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>Kl563/34-1</GrantID><Agency>Deutsche Forschungsgemeinschaft</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Photosynth Res</MedlineTA><NlmUniqueID>100954728</NlmUniqueID><ISSNLinking>0166-8595</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacterial photosynthesis</Keyword><Keyword MajorTopicYN="N">Gene regulation</Keyword><Keyword MajorTopicYN="N">Iron–sulfur cluster assembly</Keyword><Keyword MajorTopicYN="N">Isc-suf operon</Keyword><Keyword MajorTopicYN="N">Promoter activities</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>08</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>16</Day><Hour>12</Hour><Minute>11</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33064275</ArticleId><ArticleId IdType="doi">10.1007/s11120-020-00789-w</ArticleId><ArticleId IdType="pii">10.1007/s11120-020-00789-w</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>République populaire de Chine</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Nie, Xin" sort="Nie, Xin" uniqKey="Nie X" first="Xin" last="Nie">Xin Nie</name></noRegion><name sortKey="Borner, Janek" sort="Borner, Janek" uniqKey="Borner J" first="Janek" last="Börner">Janek Börner</name><name sortKey="J Ger, Andreas" sort="J Ger, Andreas" uniqKey="J Ger A" first="Andreas" last="J Ger">Andreas J Ger</name><name sortKey="Klug, Gabriele" sort="Klug, Gabriele" uniqKey="Klug G" first="Gabriele" last="Klug">Gabriele Klug</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Nie, Xin" sort="Nie, Xin" uniqKey="Nie X" first="Xin" last="Nie">Xin Nie</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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